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Alternative methods

28 - 06 - 2018

An Europe into organoids

The European Union finances numerous projects in the field of organoids to accelerate scientific and medical progress. These methods do not resort to animal experimentation.
COMIET: The gut organoids available today are closed structures that should be able to open on flat surfaces. This project aims to reproduce the epithelial tissue lining the surface of the human gut. To date, the team has managed to reproduce intestinal villi on very soft materials. Eventually this project will study intestinal diseases and the toxicity of future drugs.

TOXANOID: Toxicity tests are performed on animals, short-lived primary cell cultures or tumor-derived cell lines. The goal of this project is to create organoids that indefinitely replicate primary cell cultures from human stem cells tweeked to develop into specific organs and tissue types. To date, good results have been obtained for the small intestine, colon and liver.

CLOC: Researchers are trying to produce liver tissue from human induced pluripotent stem cells (hIPSC). Hepatic organoids were thus
produced from decellularized mouse liver matrices and hIPSC. The organoids even showed hepatic microarchitectures and vascularization and couldbe used to study certain liver diseases (hereditary cholestasis). There is also the possibility of using them for  regeneration purposes.
Other projects are under way, for example, producing brain organoids or studying colon cancer.